Fig. 2

Altered differentiation of SMYD5-depleted ES cells. a Venn diagrams showing overlap between differentially expressed genes in shSmyd5 and shLuc ES cells and genes bound by OCT4, OCT4, and NANOG, or OCT4, SOX2, and NANOG. b Embryoid body (EB) formation shows abnormal differentiation of shSmyd5 ES cells. c Hematoxylin and eosin (H&E) histological sections of shLuc and shSmyd5 day 9 EBs. The arrowheads depict altered and advanced differentiation of shSmyd5 EBs relative to control (shLuc) EBs. The bottom left panel shows an EB with an atypical internal epithelial-like structure; the bottom right panel shows a thick epithelial-like layer. d H&E histological sections of teratomas generated from shLuc and shSmyd5 ES cells injected into SCID–beige mice. Tumors were harvested 4–6 weeks post-injection and evaluated using standard H&E histological methods. Transmitted white-light microscopy of sectioned teratomas. Heterogeneous differentiation of shLuc and shSmyd5 ES cells into ectoderm (keratinized epidermal cells), mesoderm (muscle and mesenchymal cells, adipocytes), and endoderm (glandular structures)