Fig. 5

H3K27me3 is enriched in the nucleus of E10.5–E15.5 male and female germ cells. a Confocal images of H3K27me3 immunofluorescence in sections of XX and XY E10.5–E11.5 bipotential gonad and E12.5–E15.5 developing ovaries and testes. Left panels are merged images: eGFP marking germ cells in green, H3K27me3 (red) and DAPI (DNA; blue). Right panels are single-channel grayscale images showing H3K27me3 staining. White arrowheads indicate Oct4-eGFP-expressing germ cells. Representative images chosen from 3 to 4 biological replicates for each time point. 10 μm scale bars. b Quantification of H3K27me3 localization in E10.5–E15.5 male and female germ cells from wild-type fetal testis and ovary sections. Percentages of cells with uniform nuclear localization (UNL; black bars) and peripheral nuclear localization (PNL; gray bars) analyzed using ImageJ Cell Counter are shown in the stacked histogram. Data represent 3–6 biological replicates for each sex, at each stage (XX/XY cells counted: n = 16/19 at E10.5; n = 44/59 at E11.5; n = 196/214 at E12.5; n = 235/213 at E13.5 and n = 176/204 at E15.5)