Fig. 3

EZH2 protein is enriched in the nucleus of E11.5–E15.5 male and female germ cells. Confocal images of EZH2 immunofluorescence in sections of XX and XY E10.5–E11.5 bipotential gonad and E12.5–E15.5 developing ovaries and testes. Left panels are merged images: eGFP marking germ cells (green), EZH2 (red) and DAPI (DNA; blue). Right panels are single-channel grayscale images showing EZH2 staining. White arrowheads indicate Oct4-eGFP-expressing germ cells. Representative images chosen from 3 to 4 biological replicates for each time point. 10 μm scale bars