Fig. 2

EED is enriched in E11.5–E12.5 male and female germ cells. Confocal images of EED immunofluorescence in sections of XX and XY E10.5–E11.5 bipotential gonad and E12.5–E15.5 developing ovaries and testes. Left panels are merged images: eGFP marking germ cells (green), EED (red) and DAPI nuclear stain (DNA; blue). Right panels are single-channel grayscale images showing EED staining. White arrowheads indicate Oct4-eGFP-expressing germ cells. Representative images chosen from 3 to 4 biological replicates for each time point. 10 μm scale bars