Fig. 11
From: Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote
Influence of G9a-specific inhibitor BIX 01294 on H3K9me2 in mouse zygotes. Zygotes were incubated with the inhibitor (5,4 μM) starting from 2 h post-fertilization until 12 h and then fixed for immunostaining. a Shown are the representative images of PN4/5 stage zygotes stained with antibodies against H3K9me2. DNA is visualized by DAPI. m Maternal pronucleus, p paternal pronucleus, pb polar body. Scale bar 50 µm. b Quantification of H3K9me2 signals, normalized against DNA signals in both parental genomes of zygotes at PN4/5. Statistical significance was calculated using t test (***P < 0.001)