Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2

Table 1 Summary of findings with regard to methylation at gametic differentially methylated regions at imprinted loci

Locus	Chr	Chromosomal region^a delineated by pyro assay	Origin^b	gDMR status^c	Sperm meth %	Somatic meth %^d	Location within gene	CpG island	Gain in 3abKO + 3a2 ESCs
H19	7	142,580,262–142,580,434	P	Known	84.19	51.06	Intergenic	No	Yes
Rasgrf1	9	89,872,365–89,872,512	P	Known	89.00	52.67	Intergenic	No	Yes
Dlk1-Gtl2 IG	12	109,528,521–109,528,661	P	Known	96.33	57.92	Intergenic	Yes	Yes
Snrpn	7	60,004,993–60,005,163	M	Known	4.41	43.42	Promoter/Exon 1	Yes	Yes
Igf2r	17	12,960,690–12,962,806	M	Known	3.00	45.71	Intronic	Yes	Yes
Peg1	6	30,687,444–30,688,524	M	Known	15.50	51.75	Intronic	Yes	Yes
Plagl1	10	13,091,014–13,091,154	M	Known	10.80	42.83	Promoter/Exon 1	Yes	Yes
Inpp5f	7	128,688,173–128,688,290	M	Known	13.10	57.19	Intronic	Yes	Yes
Grb10	11	12,025,894–12,026,044	M	Known	7.38	45.80	Intronic	Yes	No
KvDMR	7	143,295,771–143,295,910	M	Known	6.33	50.54	Intronic	Yes	No
6330408a02Rik 3′	7	13,260,963–13,261,135	M	Putative	19.43	47.71	Exon 13	Yes	No
Neurog3 upstream	10	62,127,922–62,128,093	M	Putative	15.44	43.84	Intragenic	No	Yes
FR149454 promoter	11	119,258,958–119,259,182	M	Putative	15.33	58.67	Intronic	No	Yes
Pvt1 promoter	15	62,037,136–62,037,311	M	Putative	19.42	43.30	Intragenic	No	Yes

Data are presented for each gDMR for which a validated pyrosequencing assay (pyro assay) could be established. Known gDMR are listed first and then putative, with paternal imprints preceding maternal (none of the putative gDMRs were paternal)
Chr chromosome, Origin parent of origin of methylation mark, meth methylation
^amm10 release
^bParental origin of methylation: p paternal chromosome, m maternal
^cWhether gDMR is well characterised (known) or recently discovered (putative)
^dSomatic methylation = average methylation value across three adult tissues

ISSN: 1756-8935