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Table 1 Summary of findings with regard to methylation at gametic differentially methylated regions at imprinted loci

From: Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2

Locus Chr Chromosomal regiona delineated by pyro assay Originb gDMR statusc Sperm meth % Somatic meth %d Location within gene CpG island Gain in 3abKO + 3a2 ESCs
H19 7 142,580,262–142,580,434 P Known 84.19 51.06 Intergenic No Yes
Rasgrf1 9 89,872,365–89,872,512 P Known 89.00 52.67 Intergenic No Yes
Dlk1-Gtl2 IG 12 109,528,521–109,528,661 P Known 96.33 57.92 Intergenic Yes Yes
Snrpn 7 60,004,993–60,005,163 M Known 4.41 43.42 Promoter/Exon 1 Yes Yes
Igf2r 17 12,960,690–12,962,806 M Known 3.00 45.71 Intronic Yes Yes
Peg1 6 30,687,444–30,688,524 M Known 15.50 51.75 Intronic Yes Yes
Plagl1 10 13,091,014–13,091,154 M Known 10.80 42.83 Promoter/Exon 1 Yes Yes
Inpp5f 7 128,688,173–128,688,290 M Known 13.10 57.19 Intronic Yes Yes
Grb10 11 12,025,894–12,026,044 M Known 7.38 45.80 Intronic Yes No
KvDMR 7 143,295,771–143,295,910 M Known 6.33 50.54 Intronic Yes No
6330408a02Rik 3′ 7 13,260,963–13,261,135 M Putative 19.43 47.71 Exon 13 Yes No
Neurog3 upstream 10 62,127,922–62,128,093 M Putative 15.44 43.84 Intragenic No Yes
FR149454 promoter 11 119,258,958–119,259,182 M Putative 15.33 58.67 Intronic No Yes
Pvt1 promoter 15 62,037,136–62,037,311 M Putative 19.42 43.30 Intragenic No Yes
  1. Data are presented for each gDMR for which a validated pyrosequencing assay (pyro assay) could be established. Known gDMR are listed first and then putative, with paternal imprints preceding maternal (none of the putative gDMRs were paternal)
  2. Chr chromosome, Origin parent of origin of methylation mark, meth methylation
  3. amm10 release
  4. bParental origin of methylation: p paternal chromosome, m maternal
  5. cWhether gDMR is well characterised (known) or recently discovered (putative)
  6. dSomatic methylation = average methylation value across three adult tissues