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TableĀ 5 SPOT score for ChIP-seq datasets from GM12878 (A) and mouse ES cells (B)

From: Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq

Epitope Dataset SPOT score (%)
(A) SPOT scores for GM12878 monoclonal and polyclonal datasets
H3K27ac Monoclonal Rep 1 37.42
Monoclonal Rep 2 38.86
Polyclonal ENCFF000ASP 45.34
Polyclonal ENCFF000ASU 19.41
H3K27me3 Monoclonal Rep 1 45.67
Monoclonal Rep 2 47.53
Polyclonal ENCFF000ASV 40.92
Polyclonal ENCFF000ASW 48.89
Polyclonal ENCFF000ASZ 24.35
H3K4me1 Monoclonal Rep 1 27.84
Monoclonal Rep 2 25.66
Polyclonal ENCFF000ASM 45.47
Polyclonal ENCFF000ATK 33.47
H3K4me3 Monoclonal Lot 8 Rep 1 65.41
Monoclonal Lot 8 Rep 2 67.41
Monoclonal Lot 9 Rep 1 62.18
Monoclonal Lot 9 Rep 2 58.64
Polyclonal ENCFF000ASR 27.53
Polyclonal ENCFF000AUB 16.47
H3K9me3 Monoclonal K9me3 Rep 1 25.24
Monoclonal K9me3 Rep 2 24.76
Polyclonal ENCFF000AUK 25.50
Polyclonal ENCFF000AUO 28.59
(B) SPOT scores for mouse ES cells monoclonal and polyclonal datasets
H2K27ac Monoclonal Rep1 11
Monoclonal Rep2 11
Polyclonal Mouse ENCODE Bruce4 Rep 1 16
Polyclonal Mouse ENCODE Bruce4 Rep 2 14
Polyclonal Mouse ENCODE E14 Rep 1 6
Polyclonal Mouse ENCODE E14 Rep 2 16
H3K27me3 Monoclonal Lot 1 Rep1 3
Monoclonal Lot 1 Rep2 3
Polyclonal Mouse ENCODE Bruce4 Rep 1 4
Polyclonal Mouse ENCODE Bruce4 Rep 2 4
H3K4me1 Monoclonal Rep 1 16
Monoclonal Rep 2 15
Polyclonal Mouse ENCODE Bruce4 30
Polyclonal Mouse ENCODE E14 Rep 1 24
Polyclonal Mouse ENCODE E14 Rep 2 27
H3K4me3 Monoclonal Lot 8 Rep 1 33
Monoclonal Lot 8 Rep 2 27
Monoclonal Lot 9 Rep 1 30
Monoclonal Lot 9 Rep 2 27
Polyclonal Mouse ENCODE Bruce4 Rep 1 36
Polyclonal Mouse ENCODE Bruce4 Rep 2 37
Polyclonal Mouse ENCODE E14 Rep 1 42
Polyclonal Mouse ENCODE E14 Rep 2 39
H3K9me3 Monoclonal Rep1 3
Monoclonal Rep2 3
Polyclonal Mouse ENCODE Bruce4 Rep 1 11
Polyclonal Mouse ENCODE Bruce4 Rep 2 12
  1. SPOT score is calculated as the percent of reads that overlap with peaks. For each antibody, peak calls generated by the Mouse ENCODE for the Bruce4 ES cell line (polyclonal antibody) were used to define the peak coordinates