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Table 5 SPOT score for ChIP-seq datasets from GM12878 (A) and mouse ES cells (B)

From: Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq

Epitope

Dataset

SPOT score (%)

(A) SPOT scores for GM12878 monoclonal and polyclonal datasets

H3K27ac

Monoclonal Rep 1

37.42

Monoclonal Rep 2

38.86

Polyclonal ENCFF000ASP

45.34

Polyclonal ENCFF000ASU

19.41

H3K27me3

Monoclonal Rep 1

45.67

Monoclonal Rep 2

47.53

Polyclonal ENCFF000ASV

40.92

Polyclonal ENCFF000ASW

48.89

Polyclonal ENCFF000ASZ

24.35

H3K4me1

Monoclonal Rep 1

27.84

Monoclonal Rep 2

25.66

Polyclonal ENCFF000ASM

45.47

Polyclonal ENCFF000ATK

33.47

H3K4me3

Monoclonal Lot 8 Rep 1

65.41

Monoclonal Lot 8 Rep 2

67.41

Monoclonal Lot 9 Rep 1

62.18

Monoclonal Lot 9 Rep 2

58.64

Polyclonal ENCFF000ASR

27.53

Polyclonal ENCFF000AUB

16.47

H3K9me3

Monoclonal K9me3 Rep 1

25.24

Monoclonal K9me3 Rep 2

24.76

Polyclonal ENCFF000AUK

25.50

Polyclonal ENCFF000AUO

28.59

(B) SPOT scores for mouse ES cells monoclonal and polyclonal datasets

H2K27ac

Monoclonal Rep1

11

Monoclonal Rep2

11

Polyclonal Mouse ENCODE Bruce4 Rep 1

16

Polyclonal Mouse ENCODE Bruce4 Rep 2

14

Polyclonal Mouse ENCODE E14 Rep 1

6

Polyclonal Mouse ENCODE E14 Rep 2

16

H3K27me3

Monoclonal Lot 1 Rep1

3

Monoclonal Lot 1 Rep2

3

Polyclonal Mouse ENCODE Bruce4 Rep 1

4

Polyclonal Mouse ENCODE Bruce4 Rep 2

4

H3K4me1

Monoclonal Rep 1

16

Monoclonal Rep 2

15

Polyclonal Mouse ENCODE Bruce4

30

Polyclonal Mouse ENCODE E14 Rep 1

24

Polyclonal Mouse ENCODE E14 Rep 2

27

H3K4me3

Monoclonal Lot 8 Rep 1

33

Monoclonal Lot 8 Rep 2

27

Monoclonal Lot 9 Rep 1

30

Monoclonal Lot 9 Rep 2

27

Polyclonal Mouse ENCODE Bruce4 Rep 1

36

Polyclonal Mouse ENCODE Bruce4 Rep 2

37

Polyclonal Mouse ENCODE E14 Rep 1

42

Polyclonal Mouse ENCODE E14 Rep 2

39

H3K9me3

Monoclonal Rep1

3

Monoclonal Rep2

3

Polyclonal Mouse ENCODE Bruce4 Rep 1

11

Polyclonal Mouse ENCODE Bruce4 Rep 2

12

  1. SPOT score is calculated as the percent of reads that overlap with peaks. For each antibody, peak calls generated by the Mouse ENCODE for the Bruce4 ES cell line (polyclonal antibody) were used to define the peak coordinates