Fig. 4

Maternal transmission of Wnt1DR allele does not cause silencing or CTCF binding. a Allele-specific qRT-PCR using e9.5 brains taken from four mice with maternal (DR/+) or paternal (+/DR) transmission of Wnt1DR. Data are presented as the ratio of Wnt1DR allele-specific expression to total Wnt1 expression. Heterozygous DNA (Het DNA) was used as a calibrator for the 1:2 copy number of Wnt1DR to total Wnt1. Asterisk signifies p < 0.05 using a two-tailed t test. b CTCF ChIP. Chromatin from mouse embryonic fibroblasts (MEFs) or e9.5 brain (remaining samples) was subjected to ChIP using anti-CTCF or non-immune IgG. Purified DNAs were subjected to qPCR amplifying H19 as positive control for MEF and e9.5 brain chromatin samples, an intergenic region containing no CTCF sites as a negative control for, and the Wnt1DR allele. Three biological replicates were used. Asterisk represents p < 0.05 using a two-tailed t test after accounting for multiple hypothesis testing using Bonferroni’s correction. NS not significant. Error bars denote standard deviation with error propagation