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Table 1 Properties of histone H1.0 binding to chromatin obtained with FRAP and CP

From: Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells

 

f free [%]

f short [%]

f long [%]

k on (s−1)

k off,1 (s−1)

k switch [10−3s−1]

k off,2 [10−3s−1]

Untreated

 Heterochromatin

6 ± 3

18 ± 2

76 ± 4

3.3 ± 1.7

1.05 ± 0.13

33 ± 12

8 ± 3

 Euchromatin

6 ± 3

31 ± 9

63 ± 9

4.0 ± 2.5

0.76 ± 0.21

16 ± 8

8 ± 3

TSA-treated

11 ± 4

89 ± 4

2.1 ± 1.2

0.89 ± 0.22

31 ± 11

12 ± 4

ATP-depleted

18 ± 12

82 ± 12

1.3 ± 1.0

0.89 ± 0.22

27 ± 13

12 ± 2

  1. (mean value ± standard deviation)
  2. f free—free fraction, f short—shortly bound fraction, f long—long-bound fraction, k on—association rate, k off—dissociation rate, k switch—switching rate, TSA—Trichostatin A, ATP—adenosine triphosphate
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Epigenetics & Chromatin

ISSN: 1756-8935