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Table 1 Properties of histone H1.0 binding to chromatin obtained with FRAP and CP

From: Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells

  f free [%] f short [%] f long [%] k on (s−1) k off,1 (s−1) k switch [10−3s−1] k off,2 [10−3s−1]
Untreated
 Heterochromatin 6 ± 3 18 ± 2 76 ± 4 3.3 ± 1.7 1.05 ± 0.13 33 ± 12 8 ± 3
 Euchromatin 6 ± 3 31 ± 9 63 ± 9 4.0 ± 2.5 0.76 ± 0.21 16 ± 8 8 ± 3
TSA-treated 11 ± 4 89 ± 4 2.1 ± 1.2 0.89 ± 0.22 31 ± 11 12 ± 4
ATP-depleted 18 ± 12 82 ± 12 1.3 ± 1.0 0.89 ± 0.22 27 ± 13 12 ± 2
  1. (mean value ± standard deviation)
  2. f free—free fraction, f short—shortly bound fraction, f long—long-bound fraction, k on—association rate, k off—dissociation rate, k switch—switching rate, TSA—Trichostatin A, ATP—adenosine triphosphate