Fig. 2

Observation and interpretation of chromatin dynamics seen with FCS. a MCF7 cell stably expressing H1-EGFP with typical localizations for FCS measurements used throughout this study. b Typical ACFs obtained at the different locations, showing fast decay due to free diffusion in the cytoplasm and slower decay in the nuclear and nucleolar periphery and even slower decay in euchromatin. c Different regimes of the ACFs correspond to different processes: A fast initial decay results from free H1.0 diffusion, followed by a slow decay due to chromatin-associated diffusion or relaxation, whose time constant depends on R _g . Slower processes such as photobleaching do not show up