Fig. 1

Mi2 C. elegans LET-418 and CHD-3 homologues display redundant functions in early embryonic development. a Phylogenetic tree of Mi2 homologues in different eukaryotic phyla. b Schematic structure of the C. elegans Mi2 homologues LET-418 and CHD-3. Domain abbreviations: CHDN/CT, CHD-N- or C-terminal domain; PHD, plant homology domains; CHDs, chromodomain, helicase and DNA binding; helicase domains: N- and C-terminal helicase domains, associated with DEXDc family; DUF: domain of unknown function. Mutations used in this study are indicated as deletions (black rectangles) or point mutations (asterisk). c, d Nomarski images of wild-type or chd-3(eh4) worms treated with control or let-418(RNAi), 24 h post-egg laying at 25 °C (c) or at the indicated time points (d). Scale bar 10 μm. e Redundant functions of LET-418/CHD-3 are required before the 100-cell stage. let-418(n3536) (upper graph) or let-418(n3536); chd-3(eh4) (lower graph) embryos were shifted at 25 °C to inactivate LET-418 activity at the indicated embryonic stages, and analyzed for their phenotypes. Between 19 and 33 embryos were tested per condition