Fig. 8

Regulation of Plekha1 gene expression by Pax6. a Reduced Pax6 binding affects H3K4me1 and H3K4me3 patterns at Plekha1 enhancer and promoter regions. Pax6, H3K4me1, H3K4me2, H3K4me3, and Pol II ChIP-seq signal at Plekha1 locus in cultured lens cell chromatin. The mouse Plekha1 locus is shown including the portion of the downstream Htra1 gene. The evolutionary conservation (upper track-green color) and predicted Pax6-binding sites A (see Fig. S2 for details) and B are indicated. b Expression of Plekha1 proteins in mouse embryonic eye. Note that in the lens transitional zone and other cell types, Plekha1 (red) is found in the nuclei (DAPI stainings, blue). Scale bar is 100 µm. c qRT-PCR expression of Plekha1 in the mouse E15.5 RPE of control Pax6 ^loxP/loxP and mutant Pax6 ^loxP/loxP ; DctCre demonstrated a fold change of 0.81 (pV = 0.005, n = 3). Spearman’s correlation between Pax6 and Plekha1 is 0.76 (pV = 0.037, n = 3)