Fig. 6

Analysis of gene expression in Pax6 shRNA lens cell lines. a Knockdown of Pax6 by lentivirus shRNA (sh1 and sh2). Upper panel qRT-PCR. Lower panel western immunoblot. b Overlap of Pax6-bound genes and differentially expressed genes. Differentially expressed genes were detected by RNA-seq. c qRT-PCR validation of Pax6 positively regulated genes: Cap2, Farp1, Pax6 (see a), Plekha1, Prox1, Tshz2, and Zfp536. p values <0.05 by Student’s t tests are labeled as *, while p values <0.01 were labeled as **