Fig. 2
From: Hypoxia increases genome-wide bivalent epigenetic marking by specific gain of H3K27me3
Reversible, oxygen-dependent global changes of H3K4me3 and H3K27me3 levels. a Immunoblot detection (IB) of epigenetic changes (H3K4me3 and H3K27me3) in MCF7 cells; loading control: total H3 (H3); lower panel quantification. b Ability of recombinant JMJD3 to demethylate H3K27me3 peptide in vitro was determined at 0.02, 0.2, 1, 5 and 21% (ambient) partial O2 pressure; EDTA was used as a negative control as it blocks JMJD3 activity. Number of peaks for H3K4me3 (c) and H3K27me3 (d) significantly above background level (p < 0.05) at different time points under hypoxic conditions (t = 8, t = 24 h) and after reoxygenation (t = +8 h). Bottom panels to c and d: representative gene tracks of loci displaying H3K4me3 gain (ATP2A3, GPRC5B) or H3K27me3 gain (CYP1B1, OPRL1); diamond symbol indicates direction of gene transcription