Fig. 7

BORIS is enriched at the 5′-transduced sequence at the SVA-F1 family repeats. a A schematic of tested ~4 kb sequence encompassing SVAs elements. The sequences were used to plot the average tag density of BORIS ChIP-seq in K562 cells. b BORIS is predominantly associated with VNTR repeats of SVAs, with the exception of SVA-F1 where BORIS is bound at the 5′-transduced sequence. The average tag density (tags/ten million) is shown for BORIS versus the input of ChIP-Seq data in K562 cells. c The individual genomic examples of BORIS and CTCF occupancy corresponding to b. Data were normalized to the number of mapped reads and the number of SVA elements. d SVA-F1 expression is upregulated in testis. qRT-PCR data on total testis mRNA and two BORIS-positive cancer cell lines. e KD of BORIS in K562 does not change the SVA-F1 expression. qRT-PCR data of BORIS KD by shRNA relatively to the control empty vector (EV); SVA-F1 primers were from [88]. f The induced BORIS expression in MCF7 cells slightly upregulates SVA-F1 expression. qRT-PCR for two clones expressing ectopic BORIS [55] is compared to MCF7 transfected with the vector only (EV)