Fig. 1

H3K37 is required for cell death suppression in TORC1-inhibited cells independent of histone modification status. a Spotting assay with H3WT and the indicated histone H3 mutants on control plates or plates containing 20 nM rapamycin. b As in a except a mutant that restores electrostatic charge (H3K37R) or mimics constitutive acetylation (H3K37Q) was analyzed. c H3WT and H3K37A mutants from a were spotted in parallel with a histone shuffle strain containing as the sole source of histone H3 either H3WT, H3K37A, or H3K37R. d As in a, except H3WT, H3K37A, and an H3 mutant lacking the first 32N-terminal amino acids (H3Δ1-32) were analyzed. e H3WT and H3K37A expressing control vector or vector expressing the rapamycin-resistant TOR1-1 allele were spotted to plasmid-selective media (SC-Leu) or media containing 15 nM rapamycin. f H3WT and H3K37A cells were mock treated or 20 nM rapamycin treated for the indicated times and then stained with YO-PRO-1 to detect apoptosis (Yo+ PI−) and propidium iodide to detect necrosis (Yo+ PI+). Cells were then analyzed by flow cytometry