Fig. 6

GAF-interacting factors involved in RNA-Pol pausing. a Genetic test for GAF-interacting factors. The small-eye phenotype induced by ey > GAF(2) was used to identify factors involved in GAF-mediated pausing. Each photograph represents the typical eye observed in WT and mutant backgrounds. b Detailed analysis of GAF-induced small-eye phenotype. The scanning electron microscopic graphs of typical eyes from ey > WT and ey > GAF(2) adults are shown. The total numbers of ommatidia in each eye field and the average area of each ommatidium were measured from these graphs (ey > WT, n = 12; ey > GAF(2), n = 22) and summarized below. c The effect of GAF over-expression in eye-antenna disks. Disks were stained by Hoechst 33258. The area posterior to the morphogenetic furrow (arrow) is severely reduced in ey > GAF(2) disks. d Quantitative measurement for adult eyes. The scanning electron microscopic graphs described in b were used to measure the eye field and ommatidia number separately. The average area per ommatidium was calculated from these values. e Quantitative measurement for larval imaginal disks. The nuclei density of antenna and eye disks from ey > WT and ey > GAF(2) animals was separately measured. The ratio of nuclei density between antenna and eye disks was given as A/E index. The details of the measurement are described in Additional file 1: Fig. S10A. f Effects of different mutations on small-eye phenotype. Measurement was based on the pixel number within eye perimeter in photographs taken under a dissection microscope. The average eye size was calculated from measurement of 50 eyes for each genotype. Statistical significance is indicated (t test, *p < 0.05; **p < 0.01; ***p < 0.001)