Fig. 2

Global effects of GAF on the level of RNA-Pol Ser-5p. a Reduction of RNA-Pol Ser-5p in Gaf mutants. Polytene chromosomes from WT and Gaf mutants were co-stained and processed as described in Fig. 1. b Increase in RNA-Pol Ser-5p by GAF over-expression. Polytene chromosome from dpp > WT or dpp > GAF(3) samples were co-stained as described in Fig. 1. c Detection of differential effects of GAF on RNA-Pol phosphoisoforms. Salivary gland extracts from WT, Gaf mutants and dpp > GAF(3) lines were immunoblotted with antibodies against three RNA-Pol phosphoisoforms, total RNA-Pol (ARNA3) and α-tubulin. The abundance of each RNA-Pol isoform was adjusted with that of α-tubulin. The relative abundance of each RNA-Pol isoform from triplicate experiments is shown