Fig. 9
Global hmC levels: whole-genome sequencing. a Specific levels of hmC, derived from oxidative bisulfite sequencing, were analyzed for autosomes and sex chromosomes. b hmC in the CH context was quantified in autosomes and sex chromosomes. c hmCG levels in CpG islands, shores, and shelves for all annotated CGIs per sample were combined and plotted in 200-bp bins. d In promoter regions (±2 kb of TSS, 100-bp bins), hydroxymethylation was lowest around the TSS with higher levels further upstream and downstream. e Statistical comparisons of islands as well as upstream and downstream shores and shelves revealed higher hmCG levels in shores and shelves with aging. f In promoter regions, hmCG levels were greater in 1- to 2-kb bins upstream in females with aging and 1- to 2-kb downstream of the TSS in males with aging. Two-way ANOVA, SNK post hoc *p < 0.05, **p < 0.01