Fig. 8
Global mC levels: whole-genome sequencing. Specific levels of mC were analyzed by oxidative bisulfite sequencing. a mC levels in the CG context for autosomes, X, and Y chromosomes were analyzed in young and old females and males. b In the CH context, mC levels were much lower as compared to CG. c mCG levels in CpG islands, shores, and shelves for all annotated CGIs per sample were combined and plotted in 200 bp bins. d All annotated promoter regions (±2 kb of TSS, 100-bp bins) methylation was lowest at the TSS with higher levels further upstream and downstream. e Statistical comparisons of islands as well as upstream and downstream shores and shelves revealed slightly lower levels in Old males. f For promoter regions, mCG levels in 1-kb bins upstream and downstream of the TSS were compared. Two-way ANOVA, SNK post hoc **p < 0.01