Fig. 3

DNMT3a2 mRNA expression. Four independent sets of gene expression assays were used in these studies to specifically examine (a) long (DNMT3a1), short (DNMT3a2), or both isoforms. DNMT3a2 copy number, expressed as copy number per microgram of RNA, was analyzed (b) in E11, E18, and PN1 brain and in mature (3 M) hippocampus (HP). Expression declined from a peak level at E11 to a low at 3 M that was slightly higher than background levels (No RT). DNMT3a1 copy number was analyzed for comparison in E18 brain and 3 M old hippocampus. c DNMT3a2 copy number was analyzed with Assay #1 in hippocampi from young and old, male and female Set 2 mice (n = 4/group). d Induction of DNMT3a2 by KCl, and to a lesser extent bicuculline in hippocampal embryonic cultures was determined by qPCR. e Amplification efficiency of both DNMT3a2 assays was derived from a dilution series of input cDNA and in both cases was found to be 75 %