Fig. 9

Chromatin state in hmo1∆ that leads to faster DNA end resection and faster Rad51 recruitment after DNA double-strand break is reversed on expression of H1. a Quantification of DNA resection by qRT-PCR using primers that anneal 1.6 kb upstream of the DSB. PCR products were amplified after exonuclease I treatment of genomic DNA isolated at the indicated times following DSB induction. All values were normalized to that for an independent locus (POL5). b qRT-PCR analysis of ChIP using antibody to Rad51, monitoring binding at MAT after DSB induction (galactose). Data are normalized to corresponding input control at each time point. Asterisks represent statistical significance from DDY3 at the respective time points based on Student’s t test (P < 0.05). Three independent experiments were performed