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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: The high mobility group protein HMO1 functions as a linker histone in yeast

Fig. 5

Equivalent binding of HMO1 to MAT in different growth phases. a, b Quantification by qRT-PCR of ChIP using antibody to FLAG-tagged HMO1 in DDY3, monitoring binding at the MAT locus. Data were normalized to corresponding input control. Error bars represent standard deviation of three experiments. a Cells recovering from quiescence (Rec) compared to stationary phase (Stat). b Cells in exponential phase (Exp) compared to stationary phase (Stat). Fold enrichment = ChIP/Input DNA. Three independent experiments were performed. Error bars represent standard deviation. c Growth of cells after inoculation of fresh media with stationary phase cells to OD600 ~0.05; cells were harvested for ChIP after 1 h (Rec) or 4 h (Exp)

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