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Table 1 MS-MLPA analysis of the imprinted locus Prader–Willi/Angelman (PWS/AS) on chromosome 15 for WT, WT2, FXS1 and FXS2 before and after treatment with 5-azadC (1 µM) at three different time points (T1, T3 and T8)

From: Genome-wide methylation analysis demonstrates that 5-aza-2-deoxycytidine treatment does not cause random DNA demethylation in fragile X syndrome cells

Probes WT1 UT ratio WT1 T1 ratio WT1 T3 ratio WT1 T8 ratio
15-021.5 NDN 0.48 0.30 0.25 0.59
15-022.9 SNRPN 0.56 0.43 0.51 0.48
15-022.9 SNRPN 0.57 0.35 0.41 0.45
15-022.9 SNRPN 0.58 0.31 * 0.36 * 0.5
15-022.9 SNRPN 0.41 0.18 0.3 0.22
15-023.2 UBE3A Exon 1 0 0 0 0
Probes WT2 UT ratio WT2 T1 ratio WT2 T3 ratio WT2 T8 ratio
15-021.5 NDN 0.48 0.24 0 0.37
15-022.9 SNRPN 0.52 0.4 0.47 0.61
15-022.9 SNRPN 0.52 0.39 0.44 0.55
15-022.9 SNRPN 0.5 0.36 0.47 0.57
15-022.9 SNRPN 0.41 0.19 0.26 0.32
15-023.2 UBE3A exon 1 0 0 0 0
Probes FXS1 UT ratio FXS1 T1 ratio FXS1 T3 ratio FXS1 T8 ratio
15-021.5 NDN 0.36 0.25 0.28 0.47
15-022.9 SNRPN 0.53 0.35 0.44 0.54
15-022.9 SNRPN 0.51 0.3 0.41 0.59
15-022.9 SNRPN 0.55 0.33< 0.39 0.53
15-022.9 SNRPN 0.4 0.14 0.15 0.25
15-023.2 UBE3A exon 1 0.06 0.02 0.04 0.04
Probes FXS2 UT ratio FXS2 T1 ratio FXS2 T3 ratio FXS2 T8 ratio
15-021.5 NDN 0.48 0.31 0.26 0.48
15-022.9 SNRPN 0.48 0.37 0.5 0.34
15-022.9 SNRPN 0.39 0.45 0.48 0.55
15-022.9 SNRPN 0.2 0.41 0.42 0.43
15-022.9 SNRPN 0.13 0.26 0.19 0.17
15-023.2 UBE3A exon 1 0.06 0.14 0 0
  1. Only methylation-sensitive probes are listed
  2. Note that the asterisks indicate that the magnitude of the probe ratio exceed the set of arbitrary border values. The italic values represent levels of methylation lower than 30 %, while bold italic values represent those between 30 and 70 %