Fig. 1

Mutations in residues within the globular domain of histone H3 cause lethality. a Ribbon structure of a nucleosome with histones represented in blue and globular domain histone H3 modifications shown in the colors indicated. H3 K115Ac, H3 T118p, and H3 K122Ac are located at the dyad axis, H3 K56Ac is located at the DNA entry/exit point, and H3 T80p occurs at the lateral surface of the nucleosome. b Table indicating the developmental lethal phase of the indicated Drosophila strains expressing no canonical histones (ΔHisC) or wild type (WT) or the indicated H3 mutants expressed from 12xHisGU ^VK33,27 transgenes. c Chi-squared analysis of the expected and observed frequency of emergence of flies from pupae from the progeny from the self-cross yw; ΔHisC FRT40A; 6xHisGU ^VK33,27 WT/SM5^TM6B or yw; ΔHisC FRT40A; H3mutant/SM5^TM6B. Some ΔHisC ^−/−; 12xHisGU ^VK33,27 WT are pupal lethal, so the p values indicated for the H3 mutants are normalized to wild type