Fig. 1

ERβ deficiency leads to altered DNA methylation patterns. a Histogram showing the distribution of methylation at the sequenced cytosines in wt and βerko MEFs. b Scatterplot of percentage (%) methylation in wt vs. βerko MEFs at cytosines covered in both cell types. c Pie chart presenting the genomic distribution of hypo- and hypermethylated positions. A position was considered hypermethylated if more than 80 % of the reads indicated methylation and hypomethylated if less than 20 % indicated methylation in βerko MEFs. d Enrichment (log2 ratios of observed over random) of hypo- and hypermethylated positions at different genomic features. e Comparison of regions identified by RRBS with datasets for histone modifications in MEFs [36] using GenomeInspector (Genomatix). Bar plots indicate percentages of hypomethylated (hypo) and hypermethylated (hyper) CpGs either marked by H3K4m3 (red), H3K27me3 (orange), both marks (yellow), or none of them (negative, white), or H3K4m1 (blue), H3K4m1 plus H3K27ac (green), or none of them (negative, white). Odds ratios (ORs) and p values according to Fisher exact test. f Enrichment (log2 ratios of observed over random) of histone modifications at hypo- and hypermethylated positions