Fig. 3

Heat-induced gene activation of HRDE-1 targets occurs at the transcriptional level. a Scatter plots comparing Pol II levels between 15C-G3 and each of 23C-G2, 23C-G4, 23C-G6, p15C-G1, and p15C-G2 for WT and hrde-1 mutant. b Box plot analyses of changes (a later generation vs. 15C-G3) in Pol II levels for all protein-coding genes, NHGs, and high-stringent NHGs. Later generations with significant increases (p value <0.05, Wilcoxon rank-sum test) over 15C-G3 were indicated by “*.” c Box plot analysis indicating that NHGs, particularly the high-stringent ones, are associated with high levels of H3K9me3. Wilcoxon rank-sum test was used to calculate the p values