Fig. 1
From: A continuous kinetic assay for protein and DNA methyltransferase enzymatic activities
General diagram for the continuous coupled assay for MTs activity. a In this assay, MTs activity is coupled to three enzymes: SAH nucleosidase (SAHN), adenine deaminase (ADE) and glutamate dehydrogenase to couple methyl transfer activity to NADPH oxidation. As a result, methyl transfer activity is directly monitored by a decrease in absorbance at 340Â nm. b Diagram describing the chemical structure of SAM, SAH and all other products derived from the activity of the three coupled enzyme