Fig. 1

3D-SIM recorded chromatin landscapes of nuclei representing various myelopoietic differentiation stages. Upper left panel allocation of the analyzed cell types (framed) within the myeloid differentiation pathway. Remaining panels representative xy mid-sections of DAPI stained nuclei recorded with 3D-SIM, exemplifying the transforming global nuclear landscapes during myeloid cell differentiation. A network of chromatin domain clusters (CDCs) permeated by finely branched IC channels is seen in progenitor and precursor cells. Monocytes are characterized by compacted chromatin islets formed by tight aggregations of CDCs embedded within wide IC channels. Granulocytes show a rather uniformly arranged compacted chromatin layer at the nuclear periphery around a central IC lacuna. Arrows in inset magnification point to few decondensed chromatin sites expanding from the compact chromatin layer. Scale bars 2 µm, insets 0.5 µm