Histone modifications rather than the novel regional centromeres of Zymoseptoria tritici distinguish core and accessory chromosomes

Table 1 Centromeres of Z. tritici

Chr.	Chr. length (bp)	Cen start (bp)	Cen stop (bp)	Cen length (bp)	Cen (% chr.)	AT (%)		Repeats in Cen (%)	Cen location (% chr.)
Chr.	Chr. length (bp)	Cen start (bp)	Cen stop (bp)	Cen length (bp)	Cen (% chr.)	Cen	Chr.	Repeats in Cen (%)	Cen location (% chr.)
1	6,088,797	3,839,299	3,851,749	12,450	0.20	56.3	46.9	28.2	37.0
2	3,860,111	5,12,901	5,21,916	9015	0.23	54.1	47.6	32.4	13.3
3	3,505,381	3,348,307	3,356,535	8228	0.23	50.4	47.4	0.0	4.4
4	2,880,011	2,17,113	2,26,545	9432	0.33	49.8	47.8	9.9	7.5
5	2,861,803	2,604,117	2,614,736	10,619	0.37	58.0	48.0	29.2	2.4
6	2,674,951	6,25,186	6,37,601	12,415	0.46	53.6	48.6	40.6	23.3
7	2,665,280	2,55,824	2,66,207	10,383	0.39	48.8	47.2	0.0	9.6
8	2,443,572	2,13,892	2,27,444	13,552	0.55	53.3	48.3	36.7	8.8
9	2,142,475	2,067,589	2,076,063	8474	0.40	53.6	48.5	0.5	3.0
10	1,682,575	99,716	1,09,365	9649	0.57	51.5	47.5	15.3	5.9
11	1,624,292	3,65,130	3,73,557	8427	0.52	59.8	47.2	44.2	22.5
12	1,462,624	1,80,233	1,88,209	7976	0.55	48.6	47.7	3.0	12.3
13	1,185,774	2,36,993	2,42,558	5565	0.47	47.3	48.0	0.5	19.9
14	7,73,098	59,960	70,870	10,910	1.41	46.8	51.5	28.5	7.8
15	6,39,501	3,82,500	3,94,754	12,254	1.92	50.3	49.0	18.9	40.2
16	6,07,044	3,32,004	3,42,592	10,588	1.74	57.2	48.5	36.0	37.3
17	5,84,099	4,06,958	4,18,893	11,935	2.04	57.6	48.0	26.1	29.8
18	5,73,698	1,59,000	1,71,999	12,999	2.27	51.9	50.8	3.2	27.9
19	5,49,847	1,48,227	1,59,387	11,160	2.03	54.5	48.7	0.8	27.0
20	4,72,105	94,677	1,05,169	10,492	2.22	45.9	48.5	9.5	20.1
21	4,09,213	3,40,264	3,46,657	6393	1.56	47.7	48.1	1.9	15.4

Chromosome (Chr.) length, centromere (Cen) position (Cen Start and Cen Stop), Cen length are shown. The relative sizes of centromeres as fraction of chromosome lengths (Cen % chr.) were determined by ChIP-seq analyses. Chromosomes 1 to 13 are considered core chromosomes, and Chr. 14 to 21 are considered accessory chromosomes. Note that the average size of centromeres is not different between core and accessory chromosomes. The centromeric and chromosomal AT and repeat content is shown. Note that the overall AT-content and that of the centromeric DNA between core and accessory chromosomes does not vary and that there are overall few repeats in Z. tritici centromeres. Centromeres of core chromosomes are closer to chromosome ends than those of accessory chromosomes (expressed as the percentage distance from the chromosome mid-point; Cen location as % of chromosome length)

ISSN: 1756-8935