Fig. 2

Mmi1 recruits Ccr4-Not to its mRNA targets. a Relative enrichment of Mmi1 targets in Ccr4 RIP-chip experiments (normalized to actin) in wild-type cells (left) and mmi1 mutants. Each dot corresponds to an individual mRNA, and the not1 mRNA is designated with a star. Horizontal lines the median enrichment of all Mmi1 targets. b Cell extracts were prepared from the indicated strains and mock-treated (−) or treated with RNase (+). Immunoprecipitations were carried out using antibodies against TAP. Samples were probed with antibodies against myc (top) or TAP (bottom). The interaction between TAP-Mmi1 and Red1-myc was used as a positive control. c As in B, but samples were probed with antibodies against a FLAG epitope (top) or TAP (bottom). Similar results were obtained with different epitope tag combinations of Mmi1 and Ccr4.