In PANC1 cells, treatment with ICG-001 does not affect the same genes as does reduction in levels of TCF7L2. (A) PANC1 cells were treated with siRNAs to TCF7L2 and RNA-seq was performed; see Additional file 8 for a list of genes affected by knockdown of TCF7L2. The top 1,000 differentially expressed genes after knockdown of TCF7L2 were compared to the top 1,000 genes identified to be responsive to ICG-001 in PANC1 cells. (B) Gene ontology analyses are shown for the genes commonly up- and downregulated by knockdown of TCF7L2 and treatment with ICG-001 and genes that are only affected by knockdown of TCF7L2. Terms related to the cell cycle are shown in red and terms related to the WNT pathway are shown in blue. See Additional file 9 for a complete gene ontology analysis of the different gene sets.