Effects of epigenetic inhibitors on gene expression in HCT116 cells. (A) Genes differentially expressed after treatment of HCT116 cells with ICG-001 or C646 (see Figure 2) were analyzed using Euclidean distance and K-means clustering of expression fold change. (B) Gene ontology analyses are shown for the genes commonly up- and downregulated by both drugs and for the genes that are downregulated only by one of the drugs in HCT116 cells. Terms related to the cell cycle are shown in red and terms related to WNT signaling are shown in blue. The numbers 1 to 6 in the brackets in panel A refer to different clusters that were used in the gene ontology analyses shown in panel B (see Additional file 3).