DNA analysis of ectopic CENP-A hotspots demonstrates enrichment of CpG density and transcription factor binding motifs. (A) Ectopic CENP-A associated DNA is moderately enriched in CpG motifs. Window shows CpG motifs enriched in a scanning 10 bp window from an overlay of all hotspots (B) Left: TOMTOM DNA Motif analysis of CENP-A hotspots in SW480 and HeLa cells. Right: Pie charts showing similarity of identified hotspots to known classes of transcription factor binding sites. Detailed data for analysis of hotspots can be found in Table
5. Additional file
1 contains the full list of hotspots, and a genome-wide overview is in Figure
7. (C) A list of other TOMTOM consensus motifs that correlate to the motifs identified in CENP-A hotspots includes chromatin effector proteins.