|
Array coating
|
Nitrocellulose
|
Nitrocellulose
|
Streptavidin
|
|
Peptide coverage
|
Single PTMs
|
169 peptides covering 61 sites of H3, H4, H2A and H2B
|
70 peptides covering 37 sites of H3, H4, H2A and H2B (N-term)
|
70 peptides covering 28 sites of H3, H4, H2A and H2B
|
|
Combinatorial PTMs
|
371 peptides containing up to 5 PTMs
|
309 peptides containing up to 4 PTMs
|
121 peptides containing up to 5 PTMs
|
|
Histone variants
|
134 peptides of H3, H4, H2A, H2B and H1 variants
|
—
|
4 peptides of H2A.X
|
|
Peptide length
|
13 aa
|
19 aa
|
10-20 aa
|
|
Total number of peptide species
|
674
|
379
|
191
|
|
Tiled sequence
|
✔
|
✔
|
-
|
|
Spot replicates
|
3 spots × 2 subarrays
|
1 spot × 2 subarrays
|
6 spots × 4 subarrays
|
|
Dual-channel detection
|
✔
|
-
|
✔
|
