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Table 2 Comparison between different platforms of histone peptide microarrays

From: ChIP-less analysis of chromatin states

Platforms Combinatorial PTM histone peptide microarray (this report) MODified histone peptide array (Active Motif)[7],[33] EpiGOLD histone peptide array (EpiCypher)[6],[34],[35]
Array coating Nitrocellulose Nitrocellulose Streptavidin
Peptide coverage Single PTMs 169 peptides covering 61 sites of H3, H4, H2A and H2B 70 peptides covering 37 sites of H3, H4, H2A and H2B (N-term) 70 peptides covering 28 sites of H3, H4, H2A and H2B
Combinatorial PTMs 371 peptides containing up to 5 PTMs 309 peptides containing up to 4 PTMs 121 peptides containing up to 5 PTMs
Histone variants 134 peptides of H3, H4, H2A, H2B and H1 variants — 4 peptides of H2A.X
Peptide length 13 aa 19 aa 10-20 aa
Total number of peptide species 674 379 191
Tiled sequence ✔ ✔ -
Spot replicates 3 spots × 2 subarrays 1 spot × 2 subarrays 6 spots × 4 subarrays
Dual-channel detection ✔ - ✔
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Epigenetics & Chromatin

ISSN: 1756-8935