Table 2 Comparison between different platforms of histone peptide microarrays
Platforms | Combinatorial PTM histone peptide microarray (this report) | |||
|---|---|---|---|---|
Array coating | Nitrocellulose | Nitrocellulose | Streptavidin | |
Peptide coverage | Single PTMs | 169 peptides covering 61 sites of H3, H4, H2A and H2B | 70 peptides covering 37 sites of H3, H4, H2A and H2B (N-term) | 70 peptides covering 28 sites of H3, H4, H2A and H2B |
Combinatorial PTMs | 371 peptides containing up to 5 PTMs | 309 peptides containing up to 4 PTMs | 121 peptides containing up to 5 PTMs | |
Histone variants | 134 peptides of H3, H4, H2A, H2B and H1 variants | — | 4 peptides of H2A.X | |
Peptide length | 13 aa | 19 aa | 10-20 aa | |
Total number of peptide species | 674 | 379 | 191 | |
Tiled sequence | ✔ | ✔ | - | |
Spot replicates | 3 spots × 2 subarrays | 1 spot × 2 subarrays | 6 spots × 4 subarrays | |
Dual-channel detection | ✔ | - | ✔ | |