Figure 3

The decondensed X chromatin structure in DCC-depleted worms is a result of defective compaction. (A, B) Adult RNAi treated hermaphrodite diploid nuclei stained with X-paint FISH (red) to label X chromosome territories and DAPI (blue) to label DNA. (A) Representative stained nuclei after vector RNAi treatment, dpy-30(RNAi), dpy-21(RNAi), and dpy-27(RNAi). Scale bars equal 5 μm. (B) Quantification of the percentage of nuclear volume occupied by X in vector RNAi (n = 40), dpy-30(RNAi) (n = 40), dpy-21(RNAi) (n = 40), and dpy-27(RNAi) (n = 40). Error bars indicate standard deviation. Asterisks indicate level of statistical significance by t-test analysis (three asterisks, P <0.001). (C, D) Adult hermaphrodite diploid nuclei stained with chromosome I paint FISH (red) to label chromosome I territories and DAPI (blue) to label DNA after DCC depletion. (C) Representative stained nuclei after vector RNAi treatment, dpy-30(RNAi), dpy-21(RNAi), and dpy-27(RNAi). Scale bars equal 5 μm. (D) Quantification of the percentage of nuclear volume occupied by chromosome I in vector RNAi (n = 40), dpy-30(RNAi) (n = 40), dpy-21(RNAi) (n = 40), and dpy-27(RNAi) (n = 40). Error bars indicate standard deviation.