Locus-specific dynamic DNA methylation and expression changes during dendritic cell (DC) differentiation and maturation. DNA methylation at CG sites at the promoter of SRC, PLEKHG6 and ITGB2 (A) and gene expression changes (B) were measured during the differentiation of monocyte into iDC (day 0 to day 4) and immature dendritic cell (iDC) maturation into mature dendritic cell (mDC) (day 4 to Mature day 1 to Mature day 2). iDCs without addition of maturation cocktail were grown for additional two days as well (day 5 and 6). Expression level in B was normalized to GADPH, and three biological replicates were used in each condition. One representative experiment among four replicates is shown here. Comparisons were made for Day 0 versus Day-1, Day-4 versus Mature Day-1, and Mature Day-1 versus Mature Day-2 using paired t-test. *P <0.05, **P <0.01, ***P <0.001, n.s., nonsignificant.