Immunofluorescence showing localisation of TDG between e14.5 and e17.5. TDG is stained in red, the cytoplasm of germ cells is stained for VASA in blue and nuclei are stained using sytox green (green). Images show TDG is present in germ cells between e14.5–e16.5 (A to C) but is not detectable at e17.5 (D). TDG is detectable in somatic cells throughout the time course. Scale bar = 50 μm.