Figure 5
Immunofluorescence showing localisation of 5fC between e14.5 and e17.5. One seminiferous cord is shown outlined in each image; germ cells are indicated by white arrows and somatic cells within the seminiferous cord are indicated by blue arrows. Images show that 5fC is present in germ cells between e14.5–e16.5 (A to C) but is not detectable at e17.5 (D). Propidium iodide was used as a nuclear counterstain (red). 5fC is detectable in somatic cells throughout the time course. Scale bar = 50 μm.