Skip to content

Advertisement

  • Poster presentation
  • Open Access

Genome-wide analysis reveals TET-and TDG-mediated 5-methylcytosine oxidation dynamics

  • 1,
  • 1,
  • 1,
  • 1,
  • 1,
  • 1 and
  • 1
Contributed equally
Epigenetics & Chromatin20136(Suppl 1):P75

https://doi.org/10.1186/1756-8935-6-S1-P75

Published: 18 March 2013

Keywords

  • Mouse Embryonic Stem Cell
  • Marked Accumulation
  • Comprehensive Mapping
  • Dynamic Cycling
  • Hydroxymethylcytosine

Recent studies suggest that DNA demethylation can be achieved through ten-eleven translocation (Tet) family of DNA deoxygenates mediated oxidation followed by thymine DNA glycosylase (TDG) mediated excision and repair, but it is unclear to what extent such active demethylation processes take place. Here, we generated genome-wide distribution maps of 5-methylcytosine(5mC),5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5- carboxylcytosine (5caC) in wild-type and Tdg-deficient mouse embryonic stem cells. We observe that the steady state 5fC and 5caC are preferentially detected at repetitive sequences in wild-type cells. Depletion of TDG causes marked accumulation of 5fC and 5caC at a large number of distal gene regulatory elements and transcriptionally repressed/poised gene promoters, suggesting that Tet/TDG- dependent dynamic cycling of 5mC oxidation states may be involved in regulating the function of these regions. Thus, comprehensive mapping of 5mC oxidation and BER pathway activity provides a promising approach for better understanding of DNA methylation and demethylation dynamics in development and diseases.

Notes

Authors’ Affiliations

(1)
Harvard Medical School and Boston Childrens Hospital, Boston, USA

Copyright

© Shen et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement