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  • Poster presentation
  • Open Access

Successful implementation of ChIP-seq antibody quality control at Diagenode using automated ChIP protocol on the SX-8G IP-Star® Compact

  • 2,
  • 2,
  • 1,
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Epigenetics & Chromatin20136 (Suppl 1) :P133

https://doi.org/10.1186/1756-8935-6-S1-P133

  • Published:

Keywords

  • Chromatin Immunoprecipitation
  • High Recovery
  • Biological Relevance
  • Sequencing Platform
  • Technical Hurdle

Chromatin immunoprecipitation (ChIP) is the most widely used method to study protein-DNA interactions. A successful ChIP, however, is largely depending on the use of well characterized, highly specific ChIP-grade antibodies.

ChIP-seq has become the gold standard for whole-genome mapping of protein-DNA interactions. The generalized adoption of this technology is currently limited by four main technical hurdles. First, the reproducibility and biological relevance of DNA-associated protein landscapes depend on the specificity and performance of the antibodies in the context for which they are used. Second, the ChIP-seq method requires optimized protocols ensuring high recovery and increased signal-to-noise ratio. Third, as an effort to reduce the cost per sample and improve reproducibility, the ChIP-seq method should be compatible with automation. Finally, the economical and widespread use of ChIP-seq requires access to a fast and high value/quality next-generation sequencing platform. Here, we demonstrate the successful use of the Diagenode integrated line of products to establish a QC procedure to qualify antibodies and standardize ChIP-seq experiments.

Authors’ Affiliations

(1)
Diagenode S.A., CHU, Tour GIGA B34, 3ème étage 1 Avenue de I’Hôpital, 4000 Liège, Sart-Tilman, Belgium
(2)
Diagenode Inc, 400 Morris Avenue, Suite 101, Denville, NJ 07834, USA

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