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  • Poster presentation
  • Open Access

High-resolution mapping of transcription factor binding sites on native chromatin

  • 1, 2 and
  • 1, 3
Epigenetics & Chromatin20136 (Suppl 1) :P114

https://doi.org/10.1186/1756-8935-6-S1-P114

  • Published:

Keywords

  • Binding Site
  • Transcription Factor Binding Site
  • Factor Binding Site
  • Protein Binding Site
  • Include Transcription Factor

Sequence-specific DNA-binding proteins including transcription factors (TFs) are key determinants of gene regulation and chromatin architecture. Formaldehyde cross-linking and sonication followed by chromatin immunoprecipitation and sequencing (X-ChIP-seq) is the most widely used technique for genome-wide profiling of protein binding sites. However, there are many issues associated with X-ChIP including low resolution and poor specificity and sensitivity. Here, we implement native (i.e., without cross-linking) ChIP of micrococcal nuclease-digested chromatin followed by paired-end sequencing (N-ChIP-seq) for mapping binding sites of the structurally distinct budding yeast TFs Abf1 and Reb1. N-ChIP-seq reproducibly recovers Abf1 and Reb1 binding sites with higher specificity and sensitivity than other profiling methods and identifies both previously characterized and novel sites. Altering N-ChIP-seq conditions allows flexibility in modulating specificity and sensitivity of binding site detection. Further, unlike X-ChIP methods, N-ChIP-seq is not biased toward identifying sites in accessible chromatin. Taken together, these results suggest that N-ChIP-seq outperforms current X-ChIP methodologies for genome-wide profiling of TF binding sites.

Authors’ Affiliations

(1)
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
(2)
Medical Scientist Training Program and Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA
(3)
Howard Hughes Medical Institute, Seattle, WA 98109, USA

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