Skip to content

Advertisement

  • Oral presentation
  • Open Access

Genome-wide interplay between chromatin and the transcription machinery

  • 1
Epigenetics & Chromatin20136 (Suppl 1) :O19

https://doi.org/10.1186/1756-8935-6-S1-O19

  • Published:

Keywords

  • Gene Expression
  • Transcription Factor
  • High Resolution
  • Gene Function
  • Promoter Region

With the advent of high throughput and high resolution genome-wide protein-DNA detection assays, the inter-relationships between chromatin and the transcription machinery are now becoming clearer. Here I will discuss our recent findings using MNase ChIP-seq to map nucleosome positions, and an ultra-high resolution mapping technique called ChIP-exo that we recently developed (Rhee and Pugh, Cell 2011 14:1408). What is apparent from these studies is the following: 1) transcription factors bind to many more locations in the genome than previously appreciated. 2) transcription complexes form at the interface between nucleosomes and nucleosome-free promoter regions. 3) Chromatin remodeling complexes target specific nucleosome positions, working in concert to organize nucleosomes at the beginning and end of genes. Many remodeler subunits interact asymmetrically with the nucleosome core across the genome, which may be important for the directional passage of RNA polymerase II.

Authors’ Affiliations

(1)
Penn State University, USA

Copyright

© Pugh; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement