Figure 2From: Unusual DNA packaging characteristics in endoreduplicated Caenorhabditis elegans oocytes defined by in vivo accessibility to an endogenous nuclease activityGenetic requirement for the DNase activity present in unfertilized oocytes. Genomic DNA extracted from purified oocytes ('fer-1 oocytes’) or from liquid-nitrogen-flash-frozen samples (N2 (wild type) embryos and adults of various genotypes) was radioactively-labeled using T4 polynucleotide kinase and gamma-32P-ATP, and resolved on a denaturing 12% polyacrylamide gel (8 M urea). A total of 2 μg of DNA were used for labeling and PAGE analysis for all samples except DNA from isolated fer-1(b232) oocytes (<30 ng).Back to article page