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Figure 2 | Epigenetics & Chromatin

Figure 2

From: Unusual DNA packaging characteristics in endoreduplicated Caenorhabditis elegans oocytes defined by in vivo accessibility to an endogenous nuclease activity

Figure 2

Genetic requirement for the DNase activity present in unfertilized oocytes. Genomic DNA extracted from purified oocytes ('fer-1 oocytes’) or from liquid-nitrogen-flash-frozen samples (N2 (wild type) embryos and adults of various genotypes) was radioactively-labeled using T4 polynucleotide kinase and gamma-32P-ATP, and resolved on a denaturing 12% polyacrylamide gel (8 M urea). A total of 2 μg of DNA were used for labeling and PAGE analysis for all samples except DNA from isolated fer-1(b232) oocytes (<30 ng).

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