Figure 5

Transcription through eye enhancer leads to dislodging of Zeste from the enhancer. Results of ChIP with antibodies to the Zeste protein from (A) (UAS)Ey(e)YW, (B) UAS(Ts)Ey(e)∆YtsW and (C) (EF1)Ey(e)YW transgenic lines. Diagrams summarize the results of ChIP with specific antibodies followed by real-time PCR. The ordinate shows the percentage of target sequences in the immunoprecipitated material relative to the input (10% of total crosslinked chromatin), with the genome regions for which DNA enrichment was tested being indicated on the abscissa: pUbx, promoter of the Ubx gene, positive control; ras64B, negative control; E, eye enhancer of the white gene; pW, promoter of the white gene; codW, coding part of the white gene. “P” indicates that ChIP experiments were performed with a parental transgenic line indicated above diagram; “P∆UAS”deletion of the UAS promoter; “P∆Ts”deletion of the 702-bp SV40 terminator; “P∆EF1”deletion of the EF1 promoter. Vertical lines indicate standard deviations. All ChIP experiments were performed with chromatin isolated from heads of 2-to 5-day-old males from transgenic lines homozygous for the test construct. Background immunoprecipitation (the average normalized level after chromatin treatment with a nonspecific antibody) was subtracted from normalized specific ChIP signals (obtained with specific antibodies) at each position.