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Figure 2 | Epigenetics & Chromatin

Figure 2

From: Target of rapamycin signaling regulates high mobility group protein association to chromatin, which functions to suppress necrotic cell death

Figure 2

Histone H3 mutants have differential impacts on TORC1-regulated gene expression. H3WT and the indicated histone mutants were grown to log phase and then mock treated or treated with 25 nM rapamycin for 1.5 hours. Total RNA was extracted, cDNA synthesized using random hexamer primers, and target gene expression analyzed by qPCR. The expression of all target genes was normalized to the ACT1 housekeeping gene. (A) Expression of the RNA Pol II transcribed ribosomal protein gene, RPS14B, in the indicated strains. (B) Expression of RPS14B and the RNA Pol III transcribed 5S rRNA were analyzed in H3WT and H3K4A. (C) 5S rRNA expression in the indicated histone strains. (D) Expression of the RNA Pol I transcribed ITS1-containing 35S rRNA. Individual histone H3 mutants are expressed relative to the H3 wild-type strain (H3WT), which is set to a value of 1. Data are the average and standard deviation of four independent experiments with significance determined by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.0005. Rap, rapamycin.

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Epigenetics & Chromatin

ISSN: 1756-8935