Colocalization of H2A.Z with epigenetic markers during carp seasonal acclimatization in ribosomal cistron, and L41 and Δ9-desaturase gene promoters. Sequential chromatin immunoprecipitation (ChIP-reChIP) assays showing the seasonal colocalization of H2A.Z with (A) the euchromatin marker (H4K12ac) and (B) heterochromatin marker (H3K9me3) along the ribosomal cistron. (C,D) Represent the same seasonal colocalization, but in L41 and Δ9-desaturase promoters (n = 3 for each season). Standard deviations (± SD) are shown (*P <0.005, **P <0.001, ***P = 0.019, ****P = 0.015, Student’s t-test). ChIP-reChIP, sequential chromatin immunoprecipitation; SD, standard deviation.