Nuclear content of H2A.Z from hepatocytes and pituitary cells. (A) The cross-reactivity of commercially available antibodies between H2A.Z from rat, carp, and human cells (30, 100, and 50 μg per line of nuclear extract, respectively) is shown. (B) Western blot assays of H2A.Z and H2B in hepatocytes isolated from four different fish for each season (S1, S2, S3, S4 and W1, W2, W3, W4). (C) Seasonally relative quantification of H2A.Z content. The H2A.Z content was normalized against H2B content (n = 4 for each season). Standard deviations (± SD) are shown (*P = 0.340, Student’s t-test). (D) Seasonal immunodetection of H2A.Z is shown from pituitary sections: (A to D), DNA detection with DAPI; (B to E), H2A.Z immunodetection; and (C to F), merge. Bar = 25 μm. DAPI, 4',6-diamidino-2-phenylindole; S, summer; SD, standard deviation; W, winter.