A novel methyl-binding domain protein enrichment method for identifying genome-wide tissue-specific DNA methylation from nanogram DNA samples

Table 1 Comparative DNA requirements for genome-wide methylation analysis platforms

Methodology	Starting DNA required (μg)	Platform
WGBS[6–8]	0.01–0.1	Illumina
MeDIP-Seq[3, 4]	0.05–0.3	Illumina
MBD-Seq[3]	1^a	Illumina
RRBS-Seq[3, 9]	0.01–0.05	Illumina
MRE-Seq[10]	3	Illumina
MeDIP-Chip[11]	4	Affymetrix promoter array
MBD-Chip[11]	1	Affymetrix promoter array
MRE-Chip[12]	2	User defined
Infinium Methylation BeadChip[13]	0.8	Illumina^b
CHARM[14]	10	NimbleGen
MeKL-Chip	0.02	NimbleGen/any

ISSN: 1756-8935