Table 1 Comparative DNA requirements for genome-wide methylation analysis platforms
| Methodology | Starting DNA required (μg) | Platform |
|---|---|---|
| WGBS[6–8] | 0.01–0.1 | Illumina |
| MeDIP-Seq[3, 4] | 0.05–0.3 | Illumina |
| MBD-Seq[3] | 1a | Illumina |
| RRBS-Seq[3, 9] | 0.01–0.05 | Illumina |
| MRE-Seq[10] | 3 | Illumina |
| MeDIP-Chip[11] | 4 | Affymetrix promoter array |
| MBD-Chip[11] | 1 | Affymetrix promoter array |
| MRE-Chip[12] | 2 | User defined |
| Infinium Methylation BeadChip[13] | 0.8 | Illuminab |
| CHARM[14] | 10 | NimbleGen |
| MeKL-Chip | 0.02 | NimbleGen/any |