Chemical inhibition of LSD1 increases acetylation and H3K4 methylation at the IRF1 gene. (a) ChIP of 2-PCPA treated cells and control cell lines treated with IFN-γ for 30 minutes and 90 minutes or uninduced and collected at the times indicated. An LSD1 antibody was used and qPCR quantified the precipitate yield, reported as percent of input. (b-d) ChIP of 2-PCPA treated and untreated cell lines. The indicated antibodies were used and qPCR quantified the precipitate yield, reported as percent of input. Immunoglobulin G served as the negative control. P ≤ 0.05 for 7b, c, d and crosses versus black diamonds in 7a. 2-PCPA: trans-2-phenylcyclopropylamine; ChIP: chromatin immunoprecipitation; IFN: interferon; LSD1: lysine specific histone demethylase 1 isoform a; qPCR: quantitative PCR.